Anti-C1q Antibodies in Patients with Hepatitis B Virus

08 Aug 2017 15 Sep 2017

Disclaimer:
This essay has been written and submitted by students and is not an example of our work. Please click this link to view samples of our professional work witten by our professional essay writers. Any opinions, findings, conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of EssayCompany.

Anti-C1q Antibodies in Patients with Hepatitis B Virus Infection

Ahmed Elsadek Fakhr1, Emad Abdelhamid Morad1, Marc van Ranst2, and Mahmoud Reza Pourkarim3

Abstract

Background: Hepatitis B virus (HBV) infection is associated with extrahepatic manifestations the mechanism of which is thought to be immune mediated. One of the autoantibodies accused to be associated with tissue injury in immune complex disorders is anti-C1q. This might be attributed to the ability of these autoantibodies to amplify complement activation in situ. To date, there are no data describing the prevalence of anti-C1q in patients with HBV infection.

Objectives: The aim of this study was to investigate the prevalence of anti-C1q antibodies and analyze possible associations in a population with HBV infection.

Materials and Methods: Serum samples were collected from a group of 145 patients with HBV infection and 33 apparently healthy controls. Anti-C1q antibodies were quantified by ELISA.

Results: The levels of anti-C1q antibodies showed a highly statistically significant difference between HBV cases and controls as the mean ± SD were 21.28 ± 38.72 and 6.56 ± 5.73, respectively (p< 0.001). Interestingly, cases with acute HBV infection showed higher anti-C1q levels compared with chronic HBV infection (35.37 ± 81.77 and 20.43 ± 25.60, respectively) (p= 0.031). Levels of anti-C1q antibodies were significantly higher in Belgian and Iranian population versus Egyptian population. Concerning the HBV genotype, the patients enrolled were mainly of genotypes A and D. The levels of anti-C1q antibodies were higher in genotype A (p= 0.004). Concerning the anti-C1q seropositivity, its prevalence was 22.8% among HBV cases compared with 3% among controls; however, no significant association has been found with any of the studied variables.

Conclusions: Patients with HBV infection exhibit increased production of anti-C1q antibodies. This observation may partially explain the tissue damage associated with the extrahepatic involvements of HBV.

Keywords: Anti-C1q antibodies; autoantibodies; Hepatitis; Infection

  1. Background:
  1. Objectives:

The aim of this study was to investigate the prevalence of anti-C1q antibodies and analyze possible associations in a population with HBV infection.

  1. Patients, Materials, and Methods:
    1. Ethical statement:

All procedures were conducted in accordance with the ethical principles expressed in the Declaration of Helsinki. Written informed consents were obtained from all subjects enrolled in the study.

  1. Study design and population:

The study was performed as a case control study on 2 groups. A total of 145 patients with HBV infection were enrolled in the first group. Of the 145 patients, 65, 64, and 16 were living in Iran, Belgium, and Egypt, respectively. Patients were classified into: patients with acute hepatitis B diagnosed by seropositivity for hepatitis B surface antigen (HBs-Ag) and hepatitis B core IgM (HBc-IgM), and patients with chronic hepatitis B characterized by presence of HBs-Ag and HBc-IgG. The second group included 33 apparently healthy volunteers. Patients were excluded if they had systemic lupus erythematosus (SLE) or co-infected with hepatitis C virus (HCV) or human immunodeficiency virus (HIV). One mL serum was collected from all enrolled subjects and stored at -20°C till testing.

  1. Laboratory assessment:

Anti-C1q determination in the collected serum samples was performed using commercial enzyme linked immunosorbent assay kit (QUANTA LiteTM Anti-C1q ELISA, INOVA Diagnostics, Inc., United States of America), as per the manufacturer's instructions. The samples were classified as negative, low positive, moderate positive or strong positive if the anti-C1q values were <20, 20 - 39, 40 - 80 or >80 units, respectively.

  1. Statistical analysis:

Continuous variables were expressed as the mean ± SD & median (range), and the categorical variables were expressed as a number. Continuous variables were checked for normality by using Shapiro-Wilk test. Mann Whitney U test was used to compare between two groups of non-normally distributed variables. Kruskal Wallis h test was used to compare between more than two groups of non-normally distributed variables. A p-value <0.05 was considered significant. All statistics were performed using SPSS 22.0 for windows (SPSS Inc., Chicago, IL, USA), MedCalc windows (MedCalc Software bvba 13, Ostend, Belgium) and Microsoft Office Excel 2010 for windows (Microsoft Cor., Redmond, WA, USA).    



Request Removal

If you are the real writer of this essay and no longer want to have the essay published on the our website then please click on the link below to send us request removal:

Request the removal of this essay
Get in Touch With us

Get in touch with our dedicated team to discuss about your requirements in detail. We are here to help you our best in any way. If you are unsure about what you exactly need, please complete the short enquiry form below and we will get back to you with quote as soon as possible.